CHEM 1411 |
Chemistry
I |
Section
1 |
MWF
8:00-8:50 |
|
CHEM
4223L* |
Biochemistry
I Lab |
Section
1 |
T
2:30-6:30 |
|
CHEM
4323* |
Biochemistry
II* |
Section
1 |
TTh 1:00-2:15 |
|
CHEM
5223L* |
Biochemistry
I Lab |
Section
1 |
T
2:30-6:30 |
|
CHEM
5323 |
Biochemistry
II* |
Section
1 |
TTh 1:00-2:15 |
|
CHEM
7423 |
Life
Science Biochemistry |
Section
1 |
TTh 8:00-9:15 |
|
CHEM
7423L |
Life
Science Biochemistry
Lab |
Section
1 |
Th 2:30-4:20 |
*Stacked
Courses
Educational Background:
Bachelors
of Science:
Pharmaceutical Science (
Doctoral
Degree: Chemistry (
Dissertation Title: High
Throughput Directed Enzyme Evolution Using Fluorescence Activated Cell Sorting
Supervisors: Dr. Brent Iverson (Department of Chemistry & Biochemistry) and Dr.
George Georgiou (Department of Chemical Engineering)
Postdoctoral Studies: Massachusetts Institute of Technology under
the direction of Dr. Dane Wittrup (Department of Chemical Engineering)
1. Directed Enzyme Evolution:
Directed enzyme evolution is a method to engineer enzymes and can be performed
on enzymes for which there is no structural information available. The basic
concept is the gene is target with random mutagenesis, the gene is put into a
suitable micro-organism, such as E. coli or S. cerevisiae,
the cell library is screened, and the best enzymes are isolated. The process
can then be repeated multiple times until an enzyme with the desired properties
is isolated. In my laboratory, bacterial and yeast cell surface display are
used to present the enzyme to the substrate, and then a BectonDickinson
FACSCalibur flow cytometer is used to isolate the
best enzymes at a rate of 1-2,000 cells/second.
2. Bioremediation of Manure
Lagoon Odors: In this project, S. cerevisiae are
genetically modified with enzymes to degrade the specific organic molecules
that cause foul animal odors. The concept is that these innocuous enzymes are
added to common bakers yeast, and the yeast is dumped into a manure lagoon,
initially in a test chamber in a proper biosaftey
laboratory. The yeast grow, and then degrade the
targeted organic molecules. Genetic engineering of the Bakers Yeast is
presently underway.
Publications:
9. Varadarajan N, Gam
J, Olsen MJ, Georgiou G, Iverson BL. Engineering of protease variants exhibiting high
catalytic activity and exquisite substrate selectivity. Proc Natl Acad Sci U S A 102(19):6855-60,
2005.
8. Cochran JR, Kim YS, Olsen
MJ, Bhandari R, Wittrup KD. Domain-level antibody epitope mapping through yeast surface display of epidermal
growth factor receptor fragments. J. Immun. Meth. 287(1-2):147-58,
2004.
7. Cochran JR, Kim YS, Olsen
MJ, Bhandari R, Wittrup KD. Domain-level antibody epitope mapping through yeast surface display of epidermal
growth factor receptor fragments. J Biol Chem. 279(29):30375-84, 2004.
6. Olsen MJ, Gam J, Iverson BL, Georgiou G. High-throughput FACS method
for directed evolution of substrate specificity. Methods Mol Biol. 230:
329-42, 2003.
5. Matsumara I, Olsen MJ, Ellington A. Optimization of heterologous
gene expression for in vitro
evolution. Biotechniques
30:474-6, 2001.
4. Olsen MJ, Stephens DL,
3. Olsen MJ, Iverson BL,
Georgiou G. High-Throughput Screening of Enzyme Libraries. Current Opinions in Biotechnology 11:331-7,
2000.
2. Daugherty PS, Olsen MJ,
Iverson BL, Georgiou G. Development
of an optimized expression system for the screening of antibody libraries
displayed on the Escherichia coli
surface. Protein Engineering 12:613-21,
1999.
1. Daugherty PS, Chen G, Olsen
MJ, Iverson BL, Georgiou G. Antibody
affinity maturation using bacterial surface display. Protein
Engineering 11:825-32, 1998.
American Chemical Society
Society of Industrial Microbiology